Superresolution Microscopy as a Percolation Problem: Maximum Achievable Imaging Density and Resolution Cost
نویسندگان
چکیده
منابع مشابه
A promising approach to molecular counting problem in superresolution microscopy.
Molecular Counting Problem in Superresolution Microscopy Cellular processes are often controlled by aggregates of protein complexes. Recent studies have witnessed that the stoichiometry, or the relative composition of protein complexes, can be dynamical (1–3). Quantifying the stoichiometry of these complexes, not only at the mean level but also its full distribution, is therefore a grand challe...
متن کاملSingle particle maximum likelihood reconstruction from superresolution microscopy images
Point localization superresolution microscopy enables fluorescently tagged molecules to be imaged beyond the optical diffraction limit, reaching single molecule localization precisions down to a few nanometers. For small objects whose sizes are few times this precision, localization uncertainty prevents the straightforward extraction of a structural model from the reconstructed images. We demon...
متن کاملStochastic approach to the molecular counting problem in superresolution microscopy.
Superresolution imaging methods--now widely used to characterize biological structures below the diffraction limit--are poised to reveal in quantitative detail the stoichiometry of protein complexes in living cells. In practice, the photophysical properties of the fluorophores used as tags in superresolution methods have posed a severe theoretical challenge toward achieving this goal. Here we d...
متن کاملA Statistical Analysis of Achievable Resolution in Incoherent Imaging
The present paper concerns the statistical analysis of limits to achievable resolution in a so-called ”diffractionlimited” imaging system. The canonical case study is that of incoherent imaging of two closely-spaced sources of possibly unequal intensities. The objective is to study how far beyond the classical Rayleigh limit of resolution one can reach at a given signal to noise ratio. We consi...
متن کاملSuperresolution imaging of biological nanostructures by spectral precision distance microscopy.
For the improved understanding of biological systems on the nanoscale, it is necessary to enhance the resolution of light microscopy in the visible wavelength range beyond the limits of conventional epifluorescence microscopy (optical resolution of about 200 nm laterally, 600 nm axially). Recently, various far-field methods have been developed allowing a substantial increase of resolution ("sup...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2016
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.11.903